Device

Part:BBa_K206011:Design

Designed by: Amelia Hardjasa   Group: iGEM09_British_Columbia   (2009-10-19)


Jammer proof of concept (J23101)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 110
    Illegal NheI site found at 133
    Illegal NheI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 808


Design Notes

GFP is constitutively transcribed and expressed from the constitutive promoter BBa_J23101(relative strength 1791). Upon the addition of arabinose, pBAD induces transcription of a reverse transcript. Binding of the reverse and forward transcript result in knockdown (i.e. decreased expression) of reporter gene transcription, either through signaling for degradation or simple hybridization preventing translation of the mRNA.


Source

Assembled from Registry BioBricks.

References